Rat DRG neurons are cultured on a MED Probe (4 days in culture) and changes of spontaneous firings with temperature variation and administration of Capsaicin is observed.

A: a: Spontaneous firing recorded from 1 electrode at 37 ̊ and 43.8 ̊C. b: Raster plots for distribution of extracted spikes across multiple selected electrodes. Spike frequencies increase as temperature rises.

B: a: Spontaneous firing recorded from 1 electrode before and after administration of Capsaicin (100 nM). b: Raster plots for distribution of extracted spikes across multiple selected electrodes. Capsaicin increases firing rate remarkably.